Composite

Part:BBa_K1635000:Design

Designed by: Rebecca Buchanan   Group: iGEM15_UGA-Georgia   (2015-09-18)


BBa_K1635000 (Archaeal translational efficiency part)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have ensured Bio Brick Compatibility for all our parts.


Source

The PhmvA promoter is a known archaeal promoter, the native RBS sequence was obtained from Methanococcus voltae. The mCherry gene was obtained from Clonentech (Discosoma sp.)and was then codon optimized for expression in Methanococcus.

References